Effect of Different Concentrations of White Tea Extract on the Quality of Awassi Ram Semen on Chilled Storage
Keywords:
white tea, antioxidants, ram semen, extender, chillingAbstract
The white tea extract (WTE) has compounds that act as antioxidants, which enhance sperm characteristics during storage. The present study was aimed at investigating the effect of adding different concentrations of WTE stored at 4 °C for 24, 48 and 72 h on semen quality. Semen samples were collected from three Awassi rams, and the ejaculates were combined. Semen were divided into four equal parts, and it was diluted with Tris; beside the extender, the control group was (A0, without WTE), the second group A1 (0.05 mg WTE), the third group was A2 (0.10 mg WTE), and the fourth group was A3 (0.15 mg WTE). Results revealed the percentages of individual motility, live, and sperm membrane integrity were enhanced significantly (P <0.05) throughout storage in the A2 group compared to other groups. The A1, A2 and A3 groups decreased significantly (P <0.05) in total sperm abnormality percent at 48 and 72 h. Abnormal head sperm decreased significantly (P <0.05) at 24, 48 and 72 h in the A2 and A3 groups. While the A2 group decreased significantly (P <0.05) in abnormal middle sperm throughout storage. In conclusion, WTE has compounds in it that function as antioxidants. Especially when 0.10 mg of WTE was added to extenders, there was an improvement in individual motility, live sperm, and sperm membrane integrity, as well as a decrease in total sperm abnormality and types of abnormal sperm (head, middle, and tail).
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