DNA Barcoding and Phylogenetic Studies of Pseudoleptonema quinquefasciatum Martynov 1935 (Hydropsychidae: Trichoptera) from India

Authors

  • Aquib Majeed Insect Systematic Research Laboratory, Department of Zoology, School of Biosciences and Biotechnology, Baba Ghulam Shah Badshah University, Rajouri, India
  • Sajad Parey Insect Systematic Research Laboratory, Department of Zoology, School of Biosciences and Biotechnology, Baba Ghulam Shah Badshah University, Rajouri, India https://orcid.org/0000-0002-2094-0812
  • Manpreet Pandher High Altitude Regional Centre, Zoological Survey of India, Saproon, Solan (H.P.), India https://orcid.org/0000-0002-8045-7267

Keywords:

DNA Barcoding, India, Phylogeny, Trichoptera

Abstract

This study initiates DNA barcoding and phylogenetic investigations of Pseudoleptonema quinquefasciatum Martynov 1935, aiming to enhance species identification, assess genetic diversity, and clarify its evolutionary relationships. By analysing mitochondrial cytochrome c oxidase subunit I (COI) gene sequences from specimens collected in the Northwest Himalaya, we successfully generated the first DNA barcode for P. quinquefasciatum from India. Our findings establish a foundational framework for future taxonomic, ecological, and conservation studies on the genetic structure of P. quinquefasciatum. The generated DNA barcode data can facilitate precise species identification, aiding in resolving cryptic diversity and potential misidentifications in closely related taxa. Furthermore, our phylogenetic analyses shed light on the evolutionary affinities of P. quinquefasciatum within Hydropsychidae, reinforcing the significance of molecular approaches in taxonomy.

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Published

2025-06-27

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Section

Animal Science