The morphological changes of oviductal mucose in oestral cycle of sows

Abstract

Article Details: Received: 2019-01-25 | Accepted: 2019-03-26 | Available online: 2019-06-30

https://doi.org/10.15414/afz.2019.22.02.34-41

The aim of this work was to describe microscopic and submicroscopic changes in uterine tube of 40 sows in the estral cycle. Samples of the uterine tube were obtained for histological studies by vivisection from three sections of uterine tube. Samples were fixed for light microscopy (LM) in formaldehyde and in glutaraldehyde paraformaldehyde for scanning (SEM) and transmission (TEM) electron microscopy. They were subsequently processed in the usual manner in the LM and electron microscopic studies laboratories. We did not detect progressive changes in the length of the uterine tube. Unlike the sows‘ weight (2.57 ± 1.12 g or 2.26 ± 0.96 g), the length of the uterine tube was virtually unchanged depending on the stage of the cycle (30.2 ± 2.51 cm in FF or 30.1 ± 2.39 cm in LF). The largest relative volume of the epithelial layer was at the follicular stage of the cycle along the entire uterine tube. The difference varied from 4.99% - isthmus to 13.62% infundibulum between each part. Significant changes were seen between the ciliary and secretory cells during the estral cycle in the various parts of the uterine tube. Ciliary cells dominated throughout the cycle in infindibulum and ampulla, whereas secretory cells in isthmus. Their changes and differentiations are the manifestations of hormonal changes that direct the estral cycle. Submicroscopic changes of cells in the estral cycle have also been described.

Keywords: sows, oviduct – uterine tube, histology

References

ABE, H. (1994) Regional variations in the ultrastructural features of secretory cells in the rat oviductal epithelium. Anatomical Record, vol. 240, no. 1, pp. 77–85.

ABE, H. and HOSHI, H. (2007) Regional and cyclic variations in the ultrastructural features of secretory cells in the oviductal epithelium of the Chinese Meishan pig. Reproduction in Domestic Animals, vol. 42, no. 3, pp. 292 – 298.

ABE, H. and HOSHI, H. (2008) Morphometric and ultrastructural changes in ciliated cells of the oviductal epithelium in prolific Chinese Meishan and Large White pigs during the oestrous cycle. Reproduction in Domestic Animals, vol. 43, no. 1, pp. 66–73.

ABE, H. and OIKAWA, T. (1992) Examination by scanning electron microscopy of oviductal epithelium of the prolific Chinese Meishan pig at follicular and luteal phases. Anatomical Record, vol. 233, no. 3, pp. 399–408.

ABE, H. et al. (1993) Scanning electron microscopy of goat oviductal epithelial cells at the follicular and luteal phases of the oestrous cycle. Journal of Anatomy, vol.183, no. 2, pp. 415–421.

ABE, H. et al. (1999) Ultrastructural features of goat oviductal secretory cells at follicular and luteal phases of the oestrous cycle. Journal of Anatomy, vol. 195, no. 4, pp. 515–521.

ABUGHRIEN, B.M. and DORE, M.A. (2000) Ciliogenesis in the uterine tube of control and superovulated heifers. Cells Tissues Organs, vol. 166, no. 4, pp. 338–48.

BESENFELDER, U. et al. (2012) Role of the Oviduct in Early Embryo Development, Reproduction in Domestic Animals, vol. 47, pp. 156–163. doi: 10.1111/j.1439-0531.2012.02070.x. ISSN 0936-6768.

BJORKMAN, N. and FREDRICSSON, B. (1961) The ultrastructure organization and the alkaline phosphatase activity of the epithelial surface of the bovine fallopian tube. Zeitschrift fur Zellforschung Und Mikroskopiche Anatomie, vol. 51, no. 5, pp. 589–596. doi:10.1007/bf00335759

BOSCH, P. and WRIGHT, R.W. (2005) The oviductal sperm reservoir in domestic mammals. Archivos de Medicina Veterinaria, vol. 37, no. 2, pp. 95–104. doi:10.4067/ s0301-732x2005000200002

BRENNER, R.M. (1969) Renewal of oviduct cilia during the menstrual cycle of rhesus monkey. Fertility and Sterility, vol. 20, no. 4, pp. 599–611. doi:10.1016/s0015-0282(16)37086-8

BULLÓN, F. et al. (1980) Ultrastructure of the oviductal mucosa of the rat. III. Basal and Peg cells. International journal of fertility, vol. 25, no. 4, pp. 293–297.

ELLINGTON, J.E. (1991) The bovine oviduct and its role in reproduction – a review of the literature. The Cornell veterinarian, vol. 81, no. 3, pp. 313–328.

HACKETT, A.J. and HAFS, H.D.(1969) Biochemical and Morphological Changes in Bovine Tubular Genitalia during the Estrous Cycle. Journal of Animal Science, vol. 29, no. 1, pp. 35–38. doi:10.2527/jas1969.29135x

HADEK, R. (1955) The secondary process in the sheep‘s oviduct. Anatomical Record, vol. 12, pp. 187 – 192.

HAFEZ, E.S.E. (1987) Reproduction in farm animals. 5th ed., Philadelphia, PA: Lea and Febiger.

KENNGOTT, R..A. and SINOWATZ F. (2007) Prenatal development of the bovine oviduct. Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C, vol. 36, no. 4, pp. 272–283. doi:10.1111/j.1439-0264.2006.00762.x

KOELLE, S. et al. (2010) Newaspects of gamete transport, fertilization,and embryonic development in the oviduct gained by means of live cell imaging. Theriogenology, vol. 73, no. 6, pp. 786–795. doi:10.1016/j.theriogenology.2009.11.002

LAUSCHOVA, I. (2003) Secretory cells and morphological manifestation of secretion in the mouse oviduct. Scripta Medica, vol. 76, no. 4, pp. 203 – 224.

LEWIS, A.W. and BERARDINELLI, J.G. (2001) Gross anatomical and histomorphometric characteristics of the oviduct and uterus during the pubertal transition in sheep. Journal of Animal Science, vol. 79, no. 1, pp. 167–175. doi:10.2527/2001.791167x

McDANIEL, J. W. et al. (1968) Influence of ovarian hormones on histology and histochemistry of the bovine oviduct. Journal of Dairy Science, vol. 51, no. 5, pp. 754–761. doi:10.3168/jds. s0022-0302(68)87067-5

MENEZO, Y. and GUERIN, P. (1997) The mammalian oviduct: biochemistry and physiology. European Journal of Obstetrics & Gynecology and Reproductive Biology, vol. 73, no. 1, pp. 99–104. doi:10.1016/s0301-2115(97)02729-2

MILLONING, G. (1962) Further observation on a phosphate buffer for osmium solution in fixation. Electron Microscopy, vol. 2, pp. 1–8. doi:10.1016/b978-1-4832-2795-5.50066-6

MRÁZ, P. and POLÓNYI, J. (1988) Metódy v elektrónovej mikroskopii živočíšnych tkanív. Bratislava: VEDA, vyd. SAV,1988.

NAKAHARI, T. et al. (2011) The regulation of ciliary beat frequency by ovarian steroids in the guinea pig Fallopian tube: interactions between oestradiol and progesterone. Biomedical Research, vol. 32, no. 5, pp. 321–328. doi:10.2220/ biomedres.32.321

NATARAJAN, T. et al. (2003) Ultrastructural studies of oviduct in buffalo (Bubalus bubalis). Indian Journal of Animal Sciences, vol. 73, pp. 522 – 523.

PRICHARD, J.F. et al. (1992) In vitro co-culture of early stage caprine embryos with oviduct and uterine epithelial cells. Human Reproduction, vol. 7, no. 4, pp. 553–557. doi:10.1093/oxfordjournals.humrep.a137689

SANGHA, G.K. et al. (2003) Changes in proteins, total lipids, cholesterol, phospholipids and phosphatase enzymes in oviductal flushings of goat. Indian Journal of Animal Sciences, vol. 73, pp. 848 – 850.

SENGER, P.L. (2003) Pathways to pregnancy and parturition. 2nd ed., New York: Current Conceptions Inc. 373 p.

SHARMA, R. K. et al. (2015) Scanning and transmission electron microscopic analysis of ampullary segment of oviduct during estrous cycle in caprines. Scanning, vol. 37, no. 1, pp. 36–41. doi:10.1002/sca.21176

SHARMA, R.K. et al. (2013) Topographic and ultrastructural variations in isthmus segment of oviduct during oestrous cycle in Caprines. Scanning, vol. 35, no. 5, pp. 344–348. doi:10.1002/sca.21073. 

SHIRISH, D. and CHAKRAVARTI, S. (2011) Manual of Obstetrics. 3rd ed., Elsevier. pp. 1–16. ISBN 9788131225561.

SHIRLEY, B. and REEDER, R.L. (1996) Cyclic changes in the ampulla of the rat oviduct. Journal of Experimental Zoology, vol. 276, no.2, pp. 164–173. doi.org/10.1002/ (SICI)1097-010X(19961001)276:2<164::AID-JEZ10>3.0.CO;2-K

STEINHAUER, N.O. et al. (2004) Morphological changes and proliferative activity in the oviductal epithelium during hormonally defined stages of oestrous cycle in the bitch. Reproduction in Domestic Animals, vol. 39, no. 2, pp. 110–119. doi:10.1111/j.1439-0531.2004.00490.x

ŠŤASTNÁ, D et al. (2013) Morphological changes of oviduct in postnatal development and in oestrous cycle of heifers. Acta Fytotechnica et Zootechnica, vol. 16, no. 4, pp. 90–98.

ŠŤASTNÝ, P. and LACKOVÁ, D. (1987) Morphological analysis of the changes in oviduct during the sexual cycle of gilts (Morfologická analýza zmien vajcovodu v pohlavnom cykle prasničiek). Živočíšna výroba, vol. 32, pp. 797–809.

TIENTHAI, P. et al. (2009) Light and scanning electron microscopic studies of oviductal epithelium in Thai swamp buffalo (Bubalus bubalis) at the follicular and luteal phases. Reproduction in Domestic Animals, vol. 44, no. 3, pp. 450–455. doi:10.1111/j.1439-0531.2008.01111.x

UHRÍN, V. (1992) Funkčná morfológia epitelov vajcovodu a maternice kravy. Bratislava: Slovak Academic Press, spol. s.r.o., ISBN 80-85665-05-0.

ULBRICH, S.E. et al. (2010) In vitro systems for intercepting early embryo-maternal cross-talk in the bovine oviduct. Theriogenology, vol. 73, no. 6, pp. 802–816. doi:10.1016/j.theriogenology.2009.09.036

VACEK, Z. (1974) Histológia a histologická technica. Martin: OSVETA.

VERHAGE, H.G. et al. (1979) Cyclic changes in ciliation, secretion and cell height of the oviductal epithelium in women. American Journal of Anatomy, vol. 156, no. 4, pp. 505–521. doi:10.1002/aja.1001560405