THE EFFECT OF DNA ISOLATION IN THE PCR ANALYSIS

Abstract

The aim of the presented study was to compare the effect of selected DNA isolation kits -  DNeasy Plant Mini Kit (QIAGEN), PowerPlant DNA Isolation Kit (MO-BIO), Isolate Plant DNA Mini Kit (BIOLINE) and the method by Rogers and Bendich (1994) in relation to the quality and purity of the final PCR products. The DNeasy Plant Mini Kit (QIAGEN) and PowerPlant DNA Isolation Kit (MO-BIO) were selected as the most suitable for DNA isolation, because of the high yield of genomic DNA. Isolated DNA was tested in PCR reactions and two types of DNA sequences were amplified - microsatellite DNA sequences and ITS (Internal Transcribed Spacers) regions. The PCR products of the highest quality amplify by the ISSR primer and primers for the ITS regions have been detected in the samples containing DNA isolated by protocol Rogers and Bendich (1994) and by isolation kit Isolate Plant DNA Mini Kit (BIOLINE).